VIM-1 Metallo-β-Lactamase-Producing Klebsiella pneumoniae Strains in Greek Hospitals

作者：Panagiota Giakkoupi, Anna Xanthaki, M. Kanelopoulou, A. Vlahaki, Vivì Miriagou, S. Kontou, E. Papafraggas, Helen Malamou-Lada, LEONIDAS S. TZOUVELEKIS, N.J. Legakis, Alkiviadis C. Vatopoulos · 发表于：Journal of Clinical Microbiology · 年份：2003 · DOI：10.1128/jcm.41.8.3893-3896.2003 · 被引用次数：169 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial biofilms and quorum sensing、Infections and bacterial resistance

Seventeen Klebsiella pneumoniae clinical isolates carrying the bla(VIM-1) metallo-beta-lactamase gene were collected in the intensive care units of three hospitals in Athens, Greece, in 2002. They exhibited various carbapenem resistance levels (Etest MICs of imipenem ranged from 4 to 32 microg/ml). All isolates gave positive results by the imipenem-EDTA synergy Etest. The isolates were classified into four main types by pulsed-field gel electrophoresis; the majority of the isolates (5 and 10 isolates) belonged to two types. The bla(VIM-1) gene cassette was part of the variable region of a class 1 integron that also included aac6, dhfrI, and aadA. This structure was carried by transferable plasmids.