Deficiency in Rhabdomyosarcomas of a Factor Required for MyoD Activity and Myogenesis

作者：Stephen J. Tapscott, Mathew J. Thayer, Harold Weintraub · 发表于：Science · 年份：1993 · DOI：10.1126/science.8383879 · 被引用次数：218 · 研究领域：RNA Research and Splicing、Molecular Biology Techniques and Applications、RNA modifications and cancer

Rhabdomyosarcoma cells express the myogenic helix-loop-helix proteins of the MyoD family but do not differentiate into skeletal muscle cells. Gel shift and transient transfection assays revealed that MyoD in the rhabdomyosarcoma cells was capable of binding DNA but was relatively nonfunctional as a transcriptional activator. Heterokaryon formation with fibroblasts resulted in the restoration of transcriptional activation by MyoD and the differentiation of the rhabdomyosarcoma cells into skeletal muscle cells. These results suggest that rhabdomyosarcomas are deficient in a factor required for MyoD activity.