Long-Range Cis Preference in DNA Homology Search Over the Length of a Drosophila Chromosome

作者：William R. Engels, Christine R Preston, Dena M. Johnson-Schlitz · 发表于：Science · 年份：1994 · DOI：10.1126/science.8128250 · 被引用次数：62 · 研究领域：DNA Repair Mechanisms、DNA and Nucleic Acid Chemistry、RNA and protein synthesis mechanisms

P element-induced chromosome breakage on the X chromosome of Drosophila melanogaster was repaired six times more frequently when a homologous template was located anywhere on the X chromosome rather than on an autosome. Cis-trans comparisons confirmed that recombinational repair was more frequent when the interacting sequences were physically connected. These results suggest that the search for homology between the broken ends and a matching template sequence occurs preferentially in the cis configuration. This cis advantage operates over more than 15 megabases of DNA.