Localization of nascent RNA and CREB binding protein with the PML-containing nuclear body

作者：Vickie J. LaMorte, Jacqueline A. Dyck, Robert Ochs, Ronald M. Evans · 发表于：Proceedings of the National Academy of Sciences · 年份：1998 · DOI：10.1073/pnas.95.9.4991 · 被引用次数：242 · 研究领域：Retinoids in leukemia and cellular processes、RNA Interference and Gene Delivery、Virus-based gene therapy research

The cellular role of the PML-containing nuclear bodies also known as ND10 or PODs remains elusive despite links to oncogenesis and viral replication. Although a potential role in transcription has been considered, direct evidence has been lacking. By developing a novel in vivo nucleic acid labeling approach, we demonstrate the existence of nascent RNA polymerase II transcripts within this nuclear body. In addition, PML and the transactivation cofactor, CREB binding protein (CBP), colocalize within the nucleus. Furthermore, we show that CBP in contrast to PML is distributed throughout the internal core of the structure. Collectively, these findings support a role for this nuclear body in transcriptional regulation.