A Method to Construct a Third-Generation Horseradish Peroxidase Biosensor: Self-Assembling Gold Nanoparticles to Three-Dimensional Sol−Gel Network

作者：Jianbo Jia, Bingquan Wang, Aiguo G. Wu, Guangjin Cheng, Zhuang Li, Shaojun Dong · 发表于：Analytical Chemistry · 年份：2002 · DOI：10.1021/ac011116w · 被引用次数：654 · 研究领域：Electrochemical sensors and biosensors、Advanced biosensing and bioanalysis techniques、Advanced Nanomaterials in Catalysis

A novel method for fabrication of horseradish peroxidase biosensor has been developed by self-assembling gold nanoparticles to a thiol-containing sol-gel network. A cleaned gold electrode was first immersed in a hydrolyzed (3-mercaptopropyl)-trimethoxysilane (MPS) sol-gel solution to assemble three-dimensional silica gel, and then gold nanoparticles were chemisorbed onto the thiol groups of the sol-gel network. Finally, horseradish peroxidase (HRP) was adsorbed onto the surface of the gold nanoparticles. The distribution of gold nanoparticles and HRP was examined by atomic force microscopy (AFM). The immobilized horseradish peroxidase exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. The performance and factors influencing the performance of the resulting biosensor were studied in detail. The resulting biosensor exhibited fast amperometric response (2.5 s) to H2O2. The detection limit of the biosensor was 2.0 micromol L(-1), and the linear range was from 5.0 micromol L(-1) to 10.0 mmol L(-1). Moreover, the studied biosensor exhibited high sensitivity, good reproducibility, and long-term stability.