High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans

作者：Siu Fai Lee, Vanessa L. White, Andrew Raymond Weeks, Ary A. Hoffmann, N. M. Endersby · 发表于：Applied and Environmental Microbiology · 年份：2012 · DOI：10.1128/aem.00069-12 · 被引用次数：139 · 研究领域：Insect symbiosis and bacterial influences、Insect and Pesticide Research、Mosquito-borne diseases and control

We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.