Life Stage and Secretory Cycle of Anterior Pituitary Basophils

作者：FUJIO YOSHIMURA, Tśuyoshi Soji, HIROKO YACHI, Hiroshi Ishikawa · 发表于：Endocrinologia Japonica · 年份：1974 · DOI：10.1507/endocrj1954.21.217 · 被引用次数：7 · 研究领域：Hypothalamic control of reproductive hormones、Ovarian function and disorders、Biotin and Related Studies

Thyrotrophs, LH-and FSH-gonadotrophs have long been universally believed to be the three independent types of basophils responsible for production of corresponding hormones. In our preparations of normal rat pituitaries stained with aldehyde thionin-PAS-orange G, however, cell type distinction among these three kinds of basophils based on the staining property was found to be almost impossible, because of the intermediate forms frequently encountered, suggesting possible transformation of basophils among these types. The LH-gonadotroph may be, in our opinion, one of the intermediate forms between thyrotrophs and FSH-gonadotrophs. The same enlarged polygonal thyrotrophlike cell was studied by optical and electron microscopy on the adjacent two slices. Their intracellular thioniophilic site corresponds with accumulation of granules on the electron microscopic level. Electron density of these granules varied from low to high in each cell, sometimes giving the picture of haloed granules. Polygonal lean basophils with a row of granules with the diameter of 100-150mμ along the plasmamembrane were identified as the immature basophils, but not as the thyrotrophs. The same ovoid LH-gonadotrophlike cell was examined by optical and electron microscopy on the adjacent two slices. According to characteristics of their fine structure, they may be nothing but the young FSH-gonadotroph-like cells with scanty round vesicles and imperfect Golgi-ring. When chromophobes dissociated and collected...