Interaction of Phalloidin with Actin

作者：Anneliese M. Lengsfeld, Irmentraut Löw, Theodor Wieland, Peter Dancker, Wilhelm Hasselbach · 发表于：Proceedings of the National Academy of Sciences · 年份：1974 · DOI：10.1073/pnas.71.7.2803 · 被引用次数：210 · 研究领域：Meat and Animal Product Quality、Bee Products Chemical Analysis、Silymarin and Mushroom Poisoning

Phalloidin, a toxic bicyclic peptide of rapid action from the toadstool, Amanita phalloides, gives rise to polymerization of G-actin to filamentous structures (Ph-actin) in a medium of low ionic strength. Ph-actin closely resembles the microfilaments found in liver membrane fractions (Ph-filaments) after in vivo or in vitro poisoning. Both phalloidin induced filaments are resistant to 0.6 M KI in contrast to F-actin, and become decorated by heavy meromyosin. After preincubation with cytochalasin B significantly fewer actin filaments are observed.