Noninvasive molecular imaging of small living subjects using Raman spectroscopy

作者：Shay Keren, Cristina L. Zavaleta, Zhen Xiang Cheng, Adam de la Zerda, Olivier S. Gheysens, Sanjiv Sam Gambhir · 发表于：Proceedings of the National Academy of Sciences · 年份：2008 · DOI：10.1073/pnas.0710575105 · 被引用次数：607 · 研究领域：Spectroscopy Techniques in Biomedical and Chemical Research、Gold and Silver Nanoparticles Synthesis and Applications、Biosensors and Analytical Detection

Molecular imaging of living subjects continues to rapidly evolve with bioluminescence and fluorescence strategies, in particular being frequently used for small-animal models. This article presents noninvasive deep-tissue molecular images in a living subject with the use of Raman spectroscopy. We describe a strategy for small-animal optical imaging based on Raman spectroscopy and Raman nanoparticles. Surface-enhanced Raman scattering nanoparticles and single-wall carbon nanotubes were used to demonstrate whole-body Raman imaging, nanoparticle pharmacokinetics, multiplexing, and in vivo tumor targeting, using an imaging system adapted for small-animal Raman imaging. The imaging modality reported here holds significant potential as a strategy for biomedical imaging of living subjects.