Direct chiral separation of caderofloxacin enantiomers by HPLC using a glycoprotein column

作者：Wei Zheng, Xu Xu, Enci Wang · 发表于：Journal of Analytical Chemistry · 年份：2006 · DOI：10.1134/s1061934806110086 · 被引用次数：5 · 研究领域：Analytical Chemistry and Chromatography、Antibiotics Pharmacokinetics and Efficacy、Microfluidic and Capillary Electrophoresis Applications

The enantiomers of caderoflxacin (CS-940), the new antibacterial fluoroquinolone compound, were separated on the commercially available α-acid glycoprotein-coated chiral stationary phase (Chiral-AGP) using the mobile phase of IPA: 0.15 M NaH 2 PO 4 + Et 3 N (pH 7.9) = 3: 97 at 0.8 mL/min with UV detection at 282 nm. The chromatographic behavior of caderofloxacin enantiomers was investigated by varying the mobile phase conditions. The chiral assay method was validated and used to determination of (R)-Caderofloxacin in (S)-Caderofloxacin raw material samples.