Rapid synthesis of oligodeoxyribonucleotides VII. Solid phase synthesis of oligodeoxyribonucleo-tides by a eontinuous flow phosphotriester method on a kieselguhr-polyamide support

作者：Michael John Gait, Hans W. D. Matthes, Mohinder Singh, Brian S. Sproat, Richard C. Titmas · 发表于：Nucleic Acids Research · 年份：1982 · DOI：10.1093/nar/10.20.6243 · 被引用次数：137 · 研究领域：Chemical Synthesis and Analysis、DNA and Nucleic Acid Chemistry、Advanced biosensing and bioanalysis techniques

A new kieselguhr-polydimethylacrylamide support has been used in a continuous flow, column system for solid phase synthesis of oligodeoxyribonucleotides by a phosphotriester procedure. Using only protected mononucleotides a 14-mer, 20-mer and 27-mer were assembled in high repetitive yield using a simple manually operated, bench top apparatus.