Growth of cultured human epidermal cells into multiple epithelia suitable for grafting.

作者：H Green, O. Kehinde, Judith M. Thomas · 发表于：Proceedings of the National Academy of Sciences · 年份：1979 · DOI：10.1073/pnas.76.11.5665 · 被引用次数：1262 · 研究领域：Wound Healing and Treatments、Corneal Surgery and Treatments、Mesenchymal stem cell research

Owing to several recent developments, the cultivability of epidermal keratinocytes, particularly those of the human, has been greatly improved. Under the conditions used, single cultured cells generate stratified colonies that ultimately fuse and form an epithelium that is reasonable approximation of the epidermis. It will be shown here that large amounts of cultured epithelium can be generated from a small piece of epidermis in a short time. We wish to bring to the attention of surgeons and cell biologists the possibility of using culture-grown epithelium derived from the same individual to restore defects in the epidermis.