Size of the Poly(A) Sequences in Encephalomyocarditis Virus RNA

作者：Marie-Lou Giron, F. Logeat, Nicole Hanania, Nicole Fossar, JOSEPH HUPPERT · 发表于：Intervirology · 年份：1975 · DOI：10.1159/000149493 · 被引用次数：8 · 研究领域：Viral Infections and Immunology Research、RNA and protein synthesis mechanisms

Poly(A) sequences were demonstrated in the RNA of encephalomyocarditis (EMC) virus by affinity chromatography on poly(U)-Sepharose, treatment with RNases, and determination of the ability of the RNA to prime for reverse transcription. The size of the poly(A) sequences was estimated as 10-20 nucleotides in length by determination of the fraction resistant to pancreatic RNases plus T1 RNases, followed by analysis of electrophoretic mobility on polyacrylamide gels and of the AMP-to-adenosine ratio after alkaline hydrolysis. The poly(A) sequences are located at the 3' end of the RNA as shown by mild digestion by snake venom phosphodiesterase.