[FeFe]-Hydrogenase-Catalyzed H 2 Production in a Photoelectrochemical Biofuel Cell

作者：Michael Hambourger, Miguel Gervaldo, Draženka Svedružić, Paul W. King, Devens Gust, Maria L. Ghirardi, Ana L. Moore, Thomas A. Moore · 发表于：Journal of the American Chemical Society · 年份：2008 · DOI：10.1021/ja077691k · 被引用次数：312 · 研究领域：Electrocatalysts for Energy Conversion、Metalloenzymes and iron-sulfur proteins、Advanced battery technologies research

The Clostridium acetobutylicum [FeFe]-hydrogenase HydA has been investigated as a hydrogen production catalyst in a photoelectrochemical biofuel cell. Hydrogenase was adsorbed to pyrolytic graphite edge and carbon felt electrodes. Cyclic voltammograms of the immobilized hydrogenase films reveal cathodic proton reduction and anodic hydrogen oxidation, with a catalytic bias toward hydrogen evolution. When corrected for the electrochemically active surface area, the cathodic current densities are similar for both carbon electrodes, and approximately 40% of those obtained with a platinum electrode. The high surface area carbon felt/hydrogenase electrode was subsequently used as the cathode in a photoelectrochemical biofuel cell. Under illumination, this device is able to oxidize a biofuel substrate and reduce protons to hydrogen. Similar photocurrents and hydrogen production rates were observed in the photoelectrochemical biofuel cell using either hydrogenase or platinum cathodes.