An Anti-Human CD13 Monoclonal Antibody That Suppresses the Suppressive Function of Treg Cells

作者：Kai Zhang, Wei Chen, Yun Zhang, Yan Ge, Songwen Ju, Songwen Ju, Peng Yang, Yongfei Tan, Zhijun Ge, Zhihong Cao, Yanpin Zhao, Haorong Wu, Songguang Ju, Songguang Ju · 发表于：Monoclonal Antibodies in Immunodiagnosis and Immunotherapy · 年份：2013 · DOI：10.1089/mab.2012.0078 · 被引用次数：4 · 研究领域：Peptidase Inhibition and Analysis、Cancer Immunotherapy and Biomarkers、Immunotherapy and Immune Responses

CD13 (CD13/aminopeptidase N, APN, or CD13/APN) is a widely expressed type II membrane-bound metalloprotease. It is often overexpressed on cancer cells and expressed on CD4(+)CD25(hi) Treg cell subpopulation with higher suppressive ability. It has been determined to be a promising target in cancer diagnosis and therapy. In this study, a functional anti-human CD13 monoclonal antibody, MAb 9E4, was obtained and the specificity of this MAb was verified by flow cytometry. This MAb effectively recognized the CD13 molecule expressed on a series of malignant cell lines. Furthermore, we demonstrated that MAb 9E4 suppresses the suppressive function of Treg cells. This functional anti-human CD13 MAb provides a valuable tool for further study targeting the CD13 positive Treg cells.