Enhanced expression of centromere protein F predicts clinical progression and prognosis in patients with prostate cancer

作者：Yangjia Zhuo, Ming Xi, Yueping Wan, Wei Hua, Yuanling Liu, Wan Song, Yulin Zhou, Hongwei Luo, Shulin Wu, Weide Zhong, Chin‐Lee Wu · 发表于：International Journal of Molecular Medicine · 年份：2015 · DOI：10.3892/ijmm.2015.2086 · 被引用次数：46 · 研究领域：Prostate Cancer Treatment and Research、Ubiquitin and proteasome pathways、Cancer Genomics and Diagnostics

Centromere protein F (CENPF) is a protein associated with the centromere-kinetochore complex and chromosomal segregation during mitosis. Previous studies have demonstrated that the upregulation of CENPF may be used as a proliferation marker of malignant cell growth in tumors. The overexpression of CENPF has also been reported to be associated with a poor prognosis in human cancers. However, the clinical significance of CENPF in prostate cancer (PCa) has not yet been fully elucidated. Thus, the aim of the present study was to determine the association of CENPF with tumor progression and prognosis in patients with PCa. The expression of CENPF at the protein level in human PCa and non-cancerous prostate tissues was detected by immunohistochemical analysis, which was further validated using a microarray-based dataset (NCBI GEO accession no: GSE21032) at the mRNA level. Subsequently, the association of CENPF expression with the clinicopathological characteristics of the patients with PCa was statistically analyzed. Immunohistochemistry and dataset analysis revealed that CENPF expression was significantly increased in the PCa tissues compared with the non-cancerous prostate tissues [immunoreactivity score (IRS): PCa, 177.98 ± 94.096 vs. benign, 121.30 ± 89.596, P < 0.001; mRNA expression in the dataset: PCa, 5.67 ± 0.47 vs. benign, 5.40 ± 0.11; P < 0.001]. Additionally, as revealed by the dataset, the upregulation of CENPF mRNA expression in the PCa tissues significantly correlated...