Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life

作者：S Thole, Daniela Kalhoefer, Sonja Voget, Martine Berger, Tim Engelhardt, Heiko Liesegang, Antje Wollherr, Staffan Kjelleberg, Rolf Daniel, Meinhard Simon, Torsten Thomas, Thorsten Brinkhoff · 发表于：The ISME Journal · 年份：2012 · DOI：10.1038/ismej.2012.62 · 被引用次数：155 · 研究领域：Microbial Community Ecology and Physiology、Genomics and Phylogenetic Studies、Bacteriophages and microbial interactions

Phaeobacter gallaeciensis, a member of the abundant marine Roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. Production of the antibiotic tropodithietic acid (TDA) makes P. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pathogens. In addition to TDA, several other secondary metabolites are produced, allowing the mutualistic bacterium to also act as an opportunistic pathogen. Here we provide the manually annotated genome sequences of the P. gallaeciensis strains DSM 17395 and 2.10, isolated at the Atlantic coast of north western Spain and near Sydney, Australia, respectively. Despite their isolation sites from the two different hemispheres, the genome comparison demonstrated a surprisingly high level of synteny (only 3% nucleotide dissimilarity and 88% and 93% shared genes). Minor differences in the genomes result from horizontal gene transfer and phage infection. Comparison of the P. gallaeciensis genomes with those of other roseobacters revealed unique genomic traits, including the production of iron-scavenging siderophores. Experiments supported the predicted capacity of both strains to grow on various algal osmolytes. Transposon mutagenesis was used to expand the current knowledge on the TDA biosynthesis pathway in strain DSM 17395. This first comparative genomic analysis of finished genomes of two closely related strains belonging to one species of the Roseobacter clade revealed features that pr...