Isolation and characterization of a bradykinin‐potentiating peptide from a bovine peptic hemoglobin hydrolysate

作者：Jean‐Marie Piot, Qiuyu Zhao, Didier Guillochon, Guy Ricart, Daniel Thomas · 发表于：FEBS Letters · 年份：1992 · DOI：10.1016/0014-5793(92)80104-o · 被引用次数：40 · 研究领域：Neuropeptides and Animal Physiology、Biochemical effects in animals、Protein Hydrolysis and Bioactive Peptides

A bradykinin potentiating peptide was isolated from a peptic bovine hemoglobin hydrolysate, by the use of reversed-phase high-performance liquid chromatography (RP-HPLC). Its primary structure, determined by fast atom bombardment (FAB) and tandem mass spectrometry (MS/MS), was identical to fragment 129-134 of the alpha-chain of bovine hemoglobin. The bradykinin potency of this peptide, as exhibited by the guinea-pig ileum contraction, was significant and comparable with some others previously described.