Targeted Gene Disruption of the 14-α Sterol Demethylase ( cyp51A ) in Aspergillus fumigatus and Its Role in Azole Drug Susceptibility

作者：Emilia Mellado, Guillermo García‐Effrón, María José Buitrago, Laura Alcázar‐Fuoli, M. Cuenca‐Estrella, J. L. Rodríguez-Tudela · 发表于：Antimicrobial Agents and Chemotherapy · 年份：2005 · DOI：10.1128/aac.49.6.2536-2538.2005 · 被引用次数：127 · 研究领域：Antifungal resistance and susceptibility、Microbial Natural Products and Biosynthesis、Peptidase Inhibition and Analysis

The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.