Regeneration of Dental-Pulp-like Tissue by Chemotaxis-Induced Cell Homing

作者：Jin Y. Kim, Xuejun Xin, Eduardo K. Moioli, Jenny Chung, Chang‐Hun Lee, Mo Chen, Susan Y. Fu, Peter D. Koch, Jeremy J. Mao · 发表于：Tissue Engineering Part A · 年份：2010 · DOI：10.1089/ten.tea.2010.0181 · 被引用次数：361 · 研究领域：Endodontics and Root Canal Treatments、Dental Trauma and Treatments、Periodontal Regeneration and Treatments

Tooth infections or injuries involving dental pulp are treated routinely by root canal therapy. Endodontically treated teeth are devitalized, susceptible to re-infections, fractures, and subsequent tooth loss. Here, we report regeneration of dental-pulp-like tissue by cell homing and without cell transplantation. Upon in vivo implantation of endodontically treated real-size, native human teeth in mouse dorsum for the tested 3 weeks, delivery of basic fibroblast growth factor and/or vascular endothelial growth factor (bFGF and/or VEGF) yielded re-cellularized and revascularized connective tissue that integrated to native dentinal wall in root canals. Further, combined delivery of bFGF, VEGF, or platelet-derived growth factor (PDGF) with a basal set of nerve growth factor (NGF) and bone morphogenetic protein-7 (BMP7) generated cellularized and vascularized tissues positive of VEGF antibody staining and apparent neo-dentin formation over the surface of native dentinal wall in some, but not all, endodontically treated teeth. Newly formed dental pulp tissue appeared dense with disconnected cells surrounded by extracellular matrix. Erythrocyte-filled blood vessels were present with endothelial-like cell lining. Reconstructed, multiple microscopic images showed complete fill of dental-pulp-like tissue in the entire root canal from root apex to pulp chamber with tissue integration to dentinal wall upon delivery of bFGF, VEGF, or PDGF with a basal set of NGF and BMP7. Quantitative ELI...