P143 Hypoxia induces hypothermia and sickness behaviour in mice following subcutaneous injection of live Staphylococcus aureus

作者：AAR Thompson, HM Marriott, Lewis J. Williams, Gary S. Shaw, Abdul G Hameed, Selina Parmar, JA Preston, Marie‐Christine Simon, Randall S. Johnson, SJ Foster, David Harold Dockrell, MK Whyte, SR Walmsley · 发表于：Thorax · 年份：2013 · DOI：10.1136/thoraxjnl-2013-204457.293 · 研究领域：Thermal Regulation in Medicine、Virus-based gene therapy research、Orthopedic Infections and Treatments

Infections frequently cause or complicate illnesses associated with hypoxaemia and local tissue hypoxia. The influence of hypoxia on the interaction between host and pathogen is therefore of considerable interest. S. aureus is a major pathogen in critical care where patients may have profound hypoxaemia and at a tissue level, S. aureus frequently infects ischaemic wounds. We investigated the effect of systemic hypoxia on host-pathogen interactions using a subcutaneous S. aureus infection model in mice. C57BL/6 mice were shaved, injected with a low dose of S. aureus (5x107 SH1000) and placed in a hypoxic chamber (10% O2) or left in room air. At 6 or 12 hours mice were assessed clinically and rectal temperature recorded. Clinical assessment of mouse sickness behaviour was made by two independent observers blinded as to which oxygen tension the mice had been exposed. Mice were anaesthetised and tissue samples (blood, skin, lung, spleen, kidney, liver and brain) obtained for analysis. Mice injected with live bacteria and placed in hypoxia developed a phenotype of sickness behaviour and hypothermia. Infected hypoxic mice had significantly higher sickness scores and lower body temperature than infected normoxic mice or hypoxic mice injected with PBS (rectal temperature at 12 hours: hypoxic 33.4˚C ± 0.74, normoxic 37.7˚C ± 0.24, hypoxic PBS-injected 38.9˚C ± 0.26, p < 0.0001). Surprisingly, we found no evidence of bacteraemia, enhanced cytokine production, vascular leak or lung inju...