Efficient Quenching of Oligomeric Fluorophores on a DNA Backbone

作者：James N. Wilson, Yin Nah Teo, Eric T. Kool · 发表于：Journal of the American Chemical Society · 年份：2007 · DOI：10.1021/ja075968a · 被引用次数：74 · 研究领域：DNA and Nucleic Acid Chemistry、Advanced biosensing and bioanalysis techniques、Luminescence and Fluorescent Materials

The quenching properties of a series of oligodeoxyribosides bearing fluorophore “bases” are described. Sequences of adjacent, π-stacked pyrenes exhibit stronger electronic interactions visible in both absorbance and emission spectra than pyrenes that are insulated by intervening adenines. Quenching by N, N ‘-dimethyl-4,4‘-bipyridinium dichloride is efficient for excimer- and exciplex-forming oligomers, with Stern−Volmer constants comparable to conjugated polymer “superquenching” schemes.