The Quantitative Measurement of DNA Hybridization from Renaturation Rates

作者：J. De Ley, H. Cattoir, A. Reynaerts · 发表于：European Journal of Biochemistry · 年份：1970 · DOI：10.1111/j.1432-1033.1970.tb00830.x · 被引用次数：2595 · 研究领域：DNA and Nucleic Acid Chemistry、Enterobacteriaceae and Cronobacter Research、DNA Repair Mechanisms

A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture. The equations, relating the degree of binding with renaturation rates, are calculated for several cases. The optimal conditions for measurements are given. The method was checked in several ways. The advantages are summarized.