Nucleotide sequence of thetetMtetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545

作者：P. Martin, Patrick Trieu‐Cuot, Patrice M. Courvalin · 发表于：Nucleic Acids Research · 年份：1986 · DOI：10.1093/nar/14.17.7047 · 被引用次数：150 · 研究领域：Salmonella and Campylobacter epidemiology、Antimicrobial Resistance in Staphylococcus、Bacteriophages and microbial interactions

The nucleotide sequence of the tetracycline resistance gene tetM encoded by streptococcal conjugative shuttle transposon Tn1545 has been determined. The resistance gene was identified as a coding sequence of 1917 base pairs corresponding to a protein with a Mr of 72,500 daltons. This value is in good agreement with that, 68,000 daltons, estimated by SDS-polyacrylamide gel electrophoresis of Escherichia coli minicell extracts. The tetM gene product does not exhibit any sequence homology with either the Gram-negative (tetA, tetB and tetC), or the Bacillus and Staphylococcus tetracycline resistance proteins. The average hydropathy value of the tetM gene product (-0.21) contrasts with those calculated for the other TET proteins which are markedly hydrophobic (0.76 to 0.93). Hybridization experiments performed with an intragenic tetM probe do not support the claim [Taylor, D. (1986), J. Bact. 165, 1037-1039)] that tetracycline resistance in Campylobacter is due to acquisition of tetM.