Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export

作者：Batool Ossareh‐Nazari, Françoise Bachelerie, Catherine Dargemont · 发表于：Science · 年份：1997 · DOI：10.1126/science.278.5335.141 · 被引用次数：723 · 研究领域：Nuclear Structure and Function、RNA Research and Splicing、Mitochondrial Function and Pathology

Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (NES). This interaction was inhibited by leptomycin B, a drug that prevents the function of the CRM1 protein in yeast. To analyze the role of the CRM1-NES interaction in nuclear export, a transport assay based on semipermeabilized cells was developed. In this system, which reconstituted NES-, cytosol-, and energy-dependent nuclear export, leptomycin B specifically blocked export of NES-containing proteins. Thus, the CRM1 protein could act as a NES receptor involved in nuclear protein export.