Novel method for evaluation of the oligomeric structure of membrane proteins

作者：Mohabir Ramjeesingh, Ling‐Jun Huan, Elizabeth Garami, Christine E. Bear · 发表于：Biochemical Journal · 年份：1999 · DOI：10.1042/bj3420119 · 被引用次数：135 · 研究领域：Lipid Membrane Structure and Behavior、Mitochondrial Function and Pathology、RNA and protein synthesis mechanisms

Assessment of the quaternary structure of membrane proteins by PAGE has been problematic owing to their relatively poor solubility in non-dissociative detergents. Here we report that several membrane proteins can be readily solubilized in their native quaternary structure with the use of the detergent perfluoro-octanoic acid (PFO). Further, PFO can be used with PAGE, thereby providing a novel, accessible tool with which to assess the molecular mass of homo-multimeric protein complexes.