Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis)

作者：Zbigniew Darżynkiewicz, Gloria Juan, Xun Li, Wojciech Gorczyca, Tomoyuki Murakami, F Traganos · 发表于：Cytometry · 年份：1997 · DOI：10.1002/(sici)1097-0320(19970101)27:1<1::aid-cyto2>3.0.co;2-l · 被引用次数：1085 · 研究领域：Cell death mechanisms and regulation、Phagocytosis and Immune Regulation、Vibrio bacteria research studies

The term cell necrobiology is introduced to comprise the life processes associated with morphological, biochemical, and molecular changes which predispose, precede, and accompany cell death, as well as the consequences and tissue response to cell death. Two alternative modes of cell death can be distinguished, apoptosis and accidental cell death, generally defined as necrosis. The wide interest in necrobiology in many disciplines stems from the realization that apoptosis, whether it occurs physiologically or as a manifestation of a pathological state, is an active mode of cell death and a subject of complex regulatory processes. A possibility exists, therefore, to interact with the regulatory machinery and thereby modulate the cell's propensity to die in response to intrinsic or exogenous signals. Flow cytometry appears to be the methodology of choice to study various aspects of necrobiology. It offers all the advantages of rapid, multiparameter analysis of large populations of individual cells to investigate the biological processes associated with cell death. Numerous methods have been developed to identify apoptotic and necrotic cells and are widely used in various disciplines, in particular in oncology and immunology. The methods based on changes in cell morphology, plasma membrane structure and transport function, function of cell organelles, DNA stability to denaturation, and endonucleolytic DNA degradation are reviewed and their applicability in the research laboratory...