Promoters ofAgrobacterium tumefaciensTi-plasmid virulence genes

作者：Anath Bandhu Das, Scott E. Stachel, Paul R. Ebert, Paul Allenza, Alice L. Montoya, Eugene W. Nester · 发表于：Nucleic Acids Research · 年份：1986 · DOI：10.1093/nar/14.3.1355 · 被引用次数：93 · 研究领域：Plant tissue culture and regeneration、CRISPR and Genetic Engineering、Bacterial Genetics and Biotechnology

The DNA sequences of the promoter and 5' upstream regions of six Agrobacterium tumefaciens Ti-plasmid encoded virulence (vir) genes were determined. The transcription initiation sites were mapped by the S1 nuclease protection assay. In the -10 region, the vir promoters share a consensus sequence that is homologous to a DNA sequence found in the same region of E. coli promoters. In contrast, the -35 region sequences are variable. Several vir genes contain two common hexanucleotide sequences, 5'CGAGTA3' and 5'GCAATT3'. Translation initiation codons for all vir genes, except virG, are preceded by sequences homologous to the ribosome binding site sequences found in E. coli.