Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization

作者：Daoguo Zhou, Mark S. Mooseker, Jorge E. Galán · 发表于：Science · 年份：1999 · DOI：10.1126/science.283.5410.2092 · 被引用次数：393 · 研究领域：Salmonella and Campylobacter epidemiology、Vibrio bacteria research studies、Escherichia coli research studies

Entry of the bacterium Salmonella typhimurium into host cells requires membrane ruffling and rearrangement of the actin cytoskeleton. Here, it is shown that the bacterial protein SipA plays a critical role in this process. SipA binds directly to actin, decreases its critical concentration, and inhibits depolymerization of actin filaments. These activities result in the spatial localization and more pronounced outward extension of the Salmonella-induced membrane ruffles, thereby facilitating bacterial uptake.