Premature Chromosome Condensation Assay for Biodosimetry; Studies with Fission-Neutrons

作者：P. G. S. Prasanna, Christopher J. Kolanko, H M Gerstenberg, William F. Blakely · 发表于：Health Physics · 年份：1997 · DOI：10.1097/00004032-199704000-00011 · 被引用次数：28 · 研究领域：Carcinogens and Genotoxicity Assessment、Radiation Effects and Dosimetry、Radiopharmaceutical Chemistry and Applications

Characterization of the premature chromosome condensation assay for radiation quality is needed. To that end, human lymphocytes were exposed in vitro to various doses of 250-kVp x rays (Y(D) = 4 keV microm(-1), Y(D) is the dose-mean lineal energy of the absorbed dose distribution, D(y), where y is defined as the energy deposited in a volume by a single event divided by the mean chord length of the volume) and to fission neutrons (Y(D) = 65 keV microm(-1)). The distribution of prematurely condensed chromosome and fragments following exposure to x rays or to neutrons were non-Poisson after repair at 37 degrees C for 24 h. Dose-response curves were constructed for the yield of excess prematurely condensed chromosome fragments as necessary for biodosimetry applications. The curves were fitted to a weighted linear model by the least-squares regression method. The neutron relative biological effectiveness (RBE) value was estimated to be 2.4 +/- 0.39.