Phase Shifting of the Circadian Clock by Induction of the Drosophila period Protein

作者：Isaac Edery, Joan E. Rutila, Michael Rosbash · 发表于：Science · 年份：1994 · DOI：10.1126/science.8284676 · 被引用次数：147 · 研究领域：Circadian rhythm and melatonin、Neurobiology and Insect Physiology Research、Light effects on plants

Virtually all organisms manifest circadian (24-hour) rhythms, governed by an ill-defined endogenous pacemaker or clock. Several lines of evidence suggest that the Drosophila melanogaster period gene product PER is a clock component. If PER were central to the time-keeping mechanism, a transient increase in its concentration would cause a stable shift in the phase of the clock. Therefore, transgenic flies bearing a heat-inducible copy of PER were subjected to temperature pulses. This treatment caused long-lasting phase shifts in the locomotor activity circadian rhythm, a result that supports the contention that PER is a bona fide clock component.