Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.

作者：G. Marbaix, G. Huez, Arsène Burny, Yvette Cleuter, Evelyne HUBERT, Madeleine LECLERCQ, Hubert Chantrenne, Hermona Soreq, Uri Nudel, Uriel Z. Littauer · 发表于：Proceedings of the National Academy of Sciences · 年份：1975 · DOI：10.1073/pnas.72.8.3065 · 被引用次数：132 · 研究领域：Animal Genetics and Reproduction

Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.