Electron microscopy of the mouse retina prepared by freeze‐substitution

作者：Anthonie Van Harreveld, F. I. Khattab · 发表于：The Anatomical Record · 年份：1968 · DOI：10.1002/ar.1091610113 · 被引用次数：18 · 研究领域：Barrier Structure and Function Studies、Connexins and lens biology、Photoreceptor and optogenetics research

Abstract The isolated retina of albino mice was fixed by freeze substitution. This method allows the electron microscopic examination of the surface layer of the tissue only. On the scleral side of the retina a considerable space was found between the outer and inner rod segments. In the spaces between the latter, microvilli of the Mueller cells seemed to float freely in the inter‐rod material. On the vitreal side extracellular space was observed in bundles of nonmyelinated fibers which are destined to form the optic nerve, and often in the inner plexiform layer. Treating the retina with 5 mM glutamate caused ballooning of elements in the plexiform layer (probably dendrites) and sometimes of the inner and outer rod segments. No obvious swelling was observed of the Mueller cells, the ganglion cells and of the nonmyelinated nerve fibers.