Osteoclast markers accumulate on cells developing from human peripheral blood mononuclear precursors

作者：Judy Faust, Dave Lacey, Pamela S. Hunt, Teresa L. Burgess, Sheila A. Scully, Gwyneth Y. Van, Alana Eli, Yixin Qian, Victoria Shalhoub · 发表于：Journal of Cellular Biochemistry · 年份：1999 · DOI：10.1002/(sici)1097-4644(19990101)72:1<67::aid-jcb8>3.0.co;2-a · 被引用次数：108 · 研究领域：Bone Metabolism and Diseases、Bone health and treatments、Bone and Dental Protein Studies

Recent studies show that human osteoclasts develop in vitro from hematopoietic cells; however, special cultures conditions and/or cytokine mobilized peripheral blood are apparently required. Here, we report that cells expressing osteoclast markers differentiate from precursors present in nonmobilized peripheral blood mononuclear cells (PBMC), without the addition of stromal cells, growth factors, cytokines or steroids; and characterize their phenotype. Three days after establishing high-density PBMC cultures (1.5 x 10(6) cells/cm2), in serum-containing medium, small adherent colonies of tartrate resistant acid phosphatase positive (TRAP+) cells emerge, amidst massive monocyte cell death. These adherent cells have an eccentrically placed, round nucleus, and express low levels of TRAP and sodium fluoride-resistant- alpha-naphthyl-acetate-esterase (NaF-R-NSE). Over the next week, this cell population accumulates phenotypic markers of osteoclasts (vitronectin receptor [VR], calcitonin receptor, TRAP, cathepsin K protein, and mRNA) with increased nuclearity, covering the entire surface by 15 days. When cultured on bone, VR+, TRAP+ cells of low multinuclearity appear and cover up to 50% of the surface. Resorption lacunae can be observed by day 22. Although these pits are not nearly as numerous as the cells of preosteoclast phenotype, they do represent the activity of a subset of osteoclast-like cells that has achieved osteoclastic maturity under these culture conditions. Transcript...