Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus

作者：Shanjun Tan, Wenkui Yu, Zhiliang Lin, Qiyi Chen, Jialiang Shi, Yi Dong, Kaipeng Duan, Xiaowu Bai, Lin Xu, Jieshou Li, Ning Li · 发表于：Mediators of Inflammation · 年份：2014 · DOI：10.1155/2014/924296 · 被引用次数：30 · 研究领域：Enhanced Recovery After Surgery、Intensive Care Unit Cognitive Disorders、Stoma care and complications

BACKGROUND: The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism. METHODS: Sprague-Dawley rats were randomized into five groups (6/group): the control group, the sham group, and three exposure groups with peritoneal air exposure for 1, 2, or 3 h. At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon. The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC). RESULTS: Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit. The length of peritoneal air exposure is correlated with the severity of both systemic and intestinal inflammations and the increases in the levels of MDA, SOD, GSH-Px, and T-AOC. CONCLUSIONS: The length of peritoneal air exposure is proportional to the degree of intestinal paralysis and the severity of intestinal inflammation, which is linked to the oxidative stress response.