Regulation of Rat Brain/HepG2 Glucose Transporter Gene Expression by Insulin and Insulin-Like Growth Factor-I in Primary Cultures of Neuronal and Glial Cells*

作者：Haim Werner, Mohan K. Raizada, Laura M. Mudd, Howard L. Foyt, Ian A. Simpson, Charles T. Roberts, Derek LeRoith · 发表于：Endocrinology · 年份：1989 · DOI：10.1210/endo-125-1-314 · 被引用次数：121 · 研究领域：Metabolism, Diabetes, and Cancer、Diet and metabolism studies、Amino Acid Enzymes and Metabolism

We have demonstrated the expression of the rat brain/HepG2 glucose transporter gene in primary cultures of rat neuronal and glial cells by Northern blot analysis with a rat brain glucose transporter cDNA probe. Incubation of both neuronal and glial cells with insulin and insulin-like growth factor-I induced a time- and dose-dependent increase in the steady state levels of glucose transporter mRNA. The maximal response was achieved between 2-4 h and subsequently decreased. Both insulin and insulin-like growth factor-I at a dose of 1 ng/ml elicited an approximately 57% increase in glucose transporter mRNA levels in neuronal cultures after 90 min, suggesting that each peptide was acting through its own receptor. On the other hand, insulin stimulated [3H]2-deoxyglucose uptake in glial, but not neuronal, cells. These results suggest that insulin-like peptides regulate the expression of the rat brain/Hep G2 glucose transporter gene at both transcriptional and posttranscriptional levels, and that these regulatory mechanisms may be dissociated from each other. Insulin-like peptides may, therefore, participate in the control of brain energy metabolism.