Biochemical and Genetic Studies of Recombination Proficiency in Escherichia coli , I. Enzymatic Activity Associated with recB + and recC + Genes

作者：Stephen D. Barbour, Alvin J. Clark · 发表于：Proceedings of the National Academy of Sciences · 年份：1970 · DOI：10.1073/pnas.65.4.955 · 被引用次数：197 · 研究领域：DNA Repair Mechanisms、DNA and Nucleic Acid Chemistry、CRISPR and Genetic Engineering

An adenosine triphosphate-dependent deoxyribonuclease activity has been detected in lysates of recB(+)recC(+) strains. Mutations in recB or recC lead to loss of this activity, suggesting that these two genes determine the nuclease activity. The over-all reaction in crude lysates digests native DNA to nucleoside monophosphates. Complementation between recB21 and recC22 in vivo leads to normal levels of ATP-dependent nuclease activity. No complementation in vitro has been detected. Mutations in a third recombination gene (recA) do not alter significantly the wild-type levels of this nuclease activity.