Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

作者：Susanna Cotecchia, Debra A. Schwinn, R R Randall, Robert J. Lefkowitz, M G Caron, Brian K. Kobilka · 发表于：Proceedings of the National Academy of Sciences · 年份：1988 · DOI：10.1073/pnas.85.19.7159 · 被引用次数：565 · 研究领域：Receptor Mechanisms and Signaling、Protein Kinase Regulation and GTPase Signaling、Advanced Proteomics Techniques and Applications

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.