Rescue of immunoglobulin secretion from human neoplastic lymphoid cells by somatic cell hybridization.

作者：Ronald Levy, Jeanette Dilley · 发表于：Proceedings of the National Academy of Sciences · 年份：1978 · DOI：10.1073/pnas.75.5.2411 · 被引用次数：131 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Chronic Lymphocytic Leukemia Research、Glycosylation and Glycoproteins Research

B leukemia cells from four different patients were hybridized with a mouse myeloma cell line with polyethylene glycol as a fusing agent. The original leukemia cells all expressed immunoglobulin on their surface, but failed to secrete it. Over 200 different human-mouse somatic cell hybrids were obtained; 57% of them secreted human immunoglobulin in large amounts. Human immunoglobulin secretion can be a stable property of these hybrid cells over months of continuous culture. In each case the human immunoglobulin secreted was restricted to the light chain type expressed by the parental B leukemia cell. In addition, these hybrid cells secreted the original mouse myeloma protein and a variety of mixed human-mouse immunoglobulin molecules.