Differential activity of recombinant colony‐stimulating factors in supporting proliferation of human peripheral blood and bone marrow myeloid progenitors in culture

作者：Daniele Caracciolo, Steven C. Clark, G Rovera · 发表于：British Journal of Haematology · 年份：1989 · DOI：10.1111/j.1365-2141.1989.tb07709.x · 被引用次数：42 · 研究领域：Hematopoietic Stem Cell Transplantation、Neutropenia and Cancer Infections、Immune cells in cancer

Unlike bone marrow progenitor cells, human myeloid progenitors isolated from peripheral blood do not form colonies in semi-solid medium in the presence of rhG-CSF, rhM-CSF or rhIL-6, but do form colonies containing neutrophils, macrophages, eosinophils, basophils or mixed neutrophilic-macrophages colonies in the presence of rhIL-3 or rhGM-CSF. Priming of blood progenitors by culturing them for several days in the presence of rhGM-CSF resulted in a dramatic increase in the frequency of cells that proliferate in response to G-CSF and IL-6 and form neutrophilic granulocytic colonies. Suspension cultures maintained in the presence of IL-3 yielded increased numbers of clonogenic cells responsive to GM-CSF and G-CSF, but not to M-CSF or IL-6. rhIL-6 did not directly stimulate colony formation of peripheral blood progenitors but did prime them to respond to G-CSF. These results are consistent with a hierarchical model of granulocytic differentiation in which circulating progenitors proceed sequentially through a programme of changing growth factor sensitivity with the following sequence: IL-3, GM-CSF, IL-6 and/or G-CSF.