Electrophoretic separation of Bacillus subtilis genes.

作者：Ronald M. Harris‐Warrick, Yehudit Elkana, Stanislav Dusko Ehrlich, Joshua Lederberg · 发表于：Proceedings of the National Academy of Sciences · 年份：1975 · DOI：10.1073/pnas.72.6.2207 · 被引用次数：91 · 研究领域：Rheology and Fluid Dynamics Studies、Enzyme Production and Characterization、Microbial Metabolic Engineering and Bioproduction

The cleavage of Bacillus subtilis DNA by EcoR1 restriction endonuclease produced segments which retain various degrees of genetic transforming activity. The active segments analyzed thus far, range in size from 23 to 3 kilobases and can be partially separated by agarose gel electrophoresis. Various markers can thus be enriched from 30- to 60-fold.