Green Fluorescent Protein as a Marker for Gene Expression

作者：Martin Chalfie, Yuan Tu, Ghia Euskirchen, William W. Ward, Douglas C. Prasher · 发表于：Science · 年份：1994 · DOI：10.1126/science.8303295 · 被引用次数：6789 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、CRISPR and Genetic Engineering、Animal Genetics and Reproduction

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.