Chicken Skin Mucosa Surrounding Adult Colorectal Adenomas is a Risk Factor for Carcinogenesis

作者：Jing-Ming Guan, Ruibo Zhao, Xueyan Zhang, Yanqiu Cheng, YU-DONG GUO, Liyan Wang, Lina Mi, Fei Liu, Xiao Ma, BaoJie Li · 发表于：American Journal of Clinical Oncology · 年份：2011 · DOI：10.1097/coc.0b013e31821dedf7 · 被引用次数：13 · 研究领域：Carcinogens and Genotoxicity Assessment、Genetic factors in colorectal cancer、Inflammatory mediators and NSAID effects

OBJECTIVES: Transformation of normal mucosa to colorectal adenoma could occur over a span of 5 to 20 years, whereas transformation of colorectal adenoma to colorectal cancer could take an additional 5 to 15 years. This study aims to investigate whether chicken skin mucosa (CSM) surrounding adult colorectal adenomas may be a risk factor for carcinogenesis. METHODS: Patients with colorectal mucosa, colorectal adenomas without CSM, or colorectal adenomas with CSM were enrolled in this study. Immunohistochemistry and enzyme-linked immunosorbent assays were used to determine the expression levels of proliferation markers (ki-67 and COX-2) and apoptosis factors (survivin and caspase-3) in tissues. RESULTS: The expression of ki-67 was significantly higher in colorectal adenomas with CSM compared with colorectal adenoma tissues (P < 0.01). Colorectal adenocarcinoma showed significantly higher levels of COX-2 protein compared with normal colorectal mucosa and colorectal adenoma tissues (P < 0.001). COX-2 expression was significantly higher in adenomas with CSM compared with normal colorectal mucosa (P < 0.001). Adenomas with CSM and adenocarcinomas exhibited significantly higher levels of survivin when compared with colorectal adenoma without CSM and normal tissues (P < 0.001). Although we found no significant difference in caspase-3 levels between adenocarcinomas and adenomas with CSM, caspase-3 expression was significantly lower in these tissues when compared with colorectal adenoma...