A Primer for Morpholino Use in Zebrafish

作者：Brent Bill, Andrew M. Petzold, Karl J. Clark, Lisa A. Schimmenti, Stephen C. Ekker · 发表于：Zebrafish · 年份：2009 · DOI：10.1089/zeb.2008.0555 · 被引用次数：472 · 研究领域：Zebrafish Biomedical Research Applications、MicroRNA in disease regulation、CRISPR and Genetic Engineering

Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.