HLA‐D Restriction of the Macrophage‐Dependent Response of Immune Human T Lymphocytes to PPD in Vitro: Inhibition by Anti‐HLA‐DR Antisera

作者：B. O. BERGHOLTZ, Erik Thorsby · 发表于：Scandinavian Journal of Immunology · 年份：1978 · DOI：10.1111/j.1365-3083.1978.tb00496.x · 被引用次数：151 · 研究领域：T-cell and B-cell Immunology、Immune Cell Function and Interaction、Cytomegalovirus and herpesvirus research

The response of T cells from sensitized individuals to low doses of PPD in vitro is macrophage-dependent. By testing different allogeneic combinations of macrophages and T lymphocytes, it was found that an optimal response required that antigen be presented by macrophages sharing at least one of the HLA-D determinants of the T cell donor. Antisera recognizing HLA-A, -B or -DR antigens were found to be able to inhibit this proliferative response. The anti-HLA-DR antisera were found to exert their inhibitory effect only when directed towards an antigen shared by the donors of the T lymphocytes and the macrophages. Anti-HLA-A and -B sera, however, had an inhibitory effect when reactive with the responding T lymphocytes, irrespective of their reactivity with the cooperating macrophages. It is concluded that an optimal secondary response of in-vivo-immunized T lymphocytes to PPD requires the combined recognition of the antigen and 'self' membrane structures encoded by the HLA-D locus.