Periodic Replication of DNA in Steadily Growing Escherichia coli: The Localized Origin of Replication

作者：Takako Nagata, Matthew S. Meselson · 发表于：Cold Spring Harbor Symposia on Quantitative Biology · 年份：1968 · DOI：10.1101/sqb.1968.033.01.062 · 被引用次数：24 · 研究领域：Bacterial Genetics and Biotechnology、DNA and Nucleic Acid Chemistry、Origins and Evolution of Life

The experiments we describe here were undertaken to learn whether successive replications of the E. coli chromosome begin at the same site, and to study certain other aspects of the pattern of replication of the bacterial chromosome during steady exponential growth. Our approach has been to investigate the distribution of time intervals that elapse between the synthesis and the replication of a short segment of the bacterial chromosome. The procedure we have adopted for determining this distribution is as follows.