An ultrastructural quantitative study of the significance of microbial multiplication during early dental plaque growth

作者：Michel Brecx, Jørgen Theilade, Rolf Attström · 发表于：Journal of Periodontal Research · 年份：1983 · DOI：10.1111/j.1600-0765.1983.tb00351.x · 被引用次数：73 · 研究领域：Oral microbiology and periodontitis research、Dental Radiography and Imaging、Periodontal Regeneration and Treatments

Plaque growth may be a result of proliferation of bacteria already present on the teeth and/or of a continuous deposition of additional salivary bacteria. The purpose of this investigation was to study the relative significance of these two mechanisms. During 4, 8, or 24 hours, plaque was allowed to form on plastic films placed on the buccal surface of the premolar 14 of subjects with healthy gingiva. In addition plastic films were used to collect plaque for an initial 4 hour period after which a filter was applied to prevent further bacterial aggregation to the deposits. The filter‐covered plastic films remained in situ for another 4 or 20 hours. After removal, the samples were processed for electron microscopy. After 4 hours, the plastic films were covered by a surface coating of an acellular material in or on which bacteria, epithelial cells, and leukocytes were observed. The 8‐hour samples exhibited the same general morphology, but contained more cellular components. After 24 hours, the plaque consisted mainly of a multilayer of bacteria. The 4‐hour samples which remained in the mouth for an additional 4‐hour period covered by a filter resembled the 8‐hour samples. Furthermore, the samples which were covered by the filter after 4 hours and allowed to remain for another 20 hours in the mouth were very similar to the 24‐hour samples. Finally, the number of bacteria present in a specified area in the section along the plastic film adjacent to the gingival margin was counted ...