K-ras gene mutations in non-small cell lung cancer in Japanese

作者：Naotaka Noda, Daisuke Matsuzoe, Toshikazu Konno, Katsunobu Kawahara, Yuichi Yamashita, Takayuki Shirakusa · 发表于：Oncology Reports · 年份：2001 · DOI：10.3892/or.8.4.889 · 被引用次数：30 · 研究领域：Lung Cancer Treatments and Mutations、PI3K/AKT/mTOR signaling in cancer、Lung Cancer Research Studies

To evaluate the association of mutations in the K-ras gene with the incidence of non-small cell lung cancer (NSCLC) in Japanese patients, 410 patients treated surgically were studied. DNA was extracted from frozen specimens, and polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) hybridization assay was used to examine K-ras mutations in codons 12, 13 and 61. K-ras mutations were found in 33 patients (8.0%), and all were smokers or ex-smokers. There were no significant differences in clinical or pathological stage between wild-type cases and mutant cases. The most frequently identified mutation was a G-->T transversion (25/33, 75.8%) that resulted in the substitution of a glycine for a cysteine or a valine. Previous studies have shown that the majority of K-ras mutations among smokers are G-->T transversion. In our study, K-ras mutations were found only in smokers, and there was a high frequency of G-->T transversions. A clear correlation exists between smoking and K-ras gene mutations.