Knockdown of Cyclophilin D Gene by RNAi Protects Rat from Ischemia/ Reperfusion-Induced Renal Injury

作者：Wei Hu, Zhiqiang Chen, Zhangqun Ye, Ding Xia, Zhongyu Xia, Jiefeng Ma, Min Zhu, Gang Chen · 发表于：Kidney & Blood Pressure Research · 年份：2010 · DOI：10.1159/000316704 · 被引用次数：17 · 研究领域：Mitochondrial Function and Pathology、Cardiac Ischemia and Reperfusion、GDF15 and Related Biomarkers

BACKGROUND/AIMS: Mitochondrial permeability transition has a critical role in ischemia/reperfusion (I/R)-induced kidney injury. It is thought that mitochondrial permeability transition occurs after the opening of the permeability transition pore, a channel which putatively consists of a voltage-dependent anion channel, adenine nucleotide translocator and cyclophilin D (CypD). Much evidence shows that CypD plays an important role in I/R-induced injury. METHODS: To evaluate the role of CypD following I/R renal injury, we tested the hypothesis that knockdown of CypD gene by RNA interference (RNAi) protects rat from I/R-induced renal injury. RESULTS: Our data show that knockdown of CypD by RNAi protects normal rat kidney cell line from hypoxia-induced necrotic death. Infection of lentivirus expressing CypD RNAi sequence produces a significant reduction of CypD at both mRNA and protein levels. Both pathologic and biochemical analyses show that knockdown of CypD by RNAi protects rat kidney from I/R-induced renal injury. CONCLUSION: Our study provides the evidence that CypD may be a potential target for protecting I/R-induced renal injury.