TERMINAL PHOSPHATE LABELED NUCLEOTIDES: SYNTHESIS, APPLICATIONS, AND LINKER EFFECT ON INCORPORATION BY DNA POLYMERASES

作者：Shiv Kumar, Anup Sood, Jeffery Wegener, Patrick J. Finn, Satyam Nampalli, John R Nelson, Anuradha Sekher, Paul G. Mitsis, John W. Macklin, Carl W. Fuller · 发表于：Nucleosides Nucleotides & Nucleic Acids · 年份：2005 · DOI：10.1081/ncn-200059823 · 被引用次数：42 · 研究领域：Molecular Biology Techniques and Applications、RNA and protein synthesis mechanisms、Advanced biosensing and bioanalysis techniques

A number of terminal phosphate-labeled nucleotides with three or more phosphates and with varied length linkers attached between the terminal phosphate and the dye have been synthesized. These nucleotides have been tested as substrates for different DNA and RNA polymerases. We have also explored their utility in DNA sequencing, SNP analysis, nucleic acid amplification, quantitative PCR, and other biochemical assays.